Human ACAT1 Recombinant Protein (RPPB5771)
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주문- SKU:
- RPPB5771
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- ACAT1
- Synonyms:
- Acetyl-CoA acetyltransferase
- Synonyms:
- mitochondrial
- Synonyms:
- EC 2319
- Synonyms:
- Acetoacetyl-CoA thiolase
- Source:
- Escherichia Coli
- Uniprot:
- P24752
Description
| Product Name: | Human ACAT1 Recombinant Protein |
| Product Code: | RPPB5771 |
| Size: | 20µg |
| Species: | Human |
| Target: | ACAT1 |
| Synonyms: | Acetyl-CoA acetyltransferase, mitochondrial, EC 2.3.1.9, Acetoacetyl-CoA thiolase, T2, ACAT1, ACAT, MAT, THIL. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The ACAT1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 7.5), 0.1M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSVSKPTLK EVVIVSATRT PIGSFLGSLS LLPATKLGSI AIQGAIEKAG IPKEEVKEAY MGNVLQGGEG QAPTRQAVLG AGLPISTPCT TINKVCASGM KAIMMASQSL MCGHQDVMVA GGMESMSNVP YVMNRGSTPY GGVKLEDLIV KDGLTDVYNKIHMGSCAENT AKKLNIARNE QDAYAINSYT RSKAAWEAGK FGNEVIPVTV TVKGQPDVVV KEDEEYKRVD FSKVPKLKTV FQKENGTVTA ANASTLNDGA AALVLMTADA AKRLNVTPLA RIVAFADAAV EPIDFPIAPV YAASMVLKDV GLKKEDIAMW EVNEAFSLVV LANIKMLEIDPQKVNINGGA VSLGHPIGMS GARIVGHLTH ALKQGEYGLA SICNGGGGAS AMLIQKL |
Acetoacetyl-CoA thiolase (ACAT1) is an enzyme member of the membrane-bound acyltransferase family and Sterol o-acyltransferase subfamily. The ACAT1 enzyme catalyzes the reversible formation of acetoacetyl-CoA from 2 molecules of acetyl-CoA. ACAT1 plays a part in lipoprotein compilation and dietary cholesterol absorption. Added to its acyltransferase activity, ACAT1 acts as a ligase.
ACAT1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 417 amino acids (34-427) and having a molecular mass of 43.8 kDa.ACAT1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ACAT1: Plays a major role in ketone body metabolism. Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD); also known as alpha- methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3- hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype. Belongs to the thiolase family. |
| UniProt Protein Details: | Protein type:Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Lipid Metabolism - fatty acid; Acetyltransferase; Lipid Metabolism - synthesis and degradation of ketone bodies; Carbohydrate Metabolism - propanoate; Amino Acid Metabolism - tryptophan; EC 2.3.1.9; Amino Acid Metabolism - lysine degradation; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - butanoate; Mitochondrial Chromosomal Location of Human Ortholog: 11q22.3 Cellular Component: mitochondrial matrix; mitochondrion Molecular Function:acetyl-CoA C-acetyltransferase activity Biological Process: branched chain family amino acid catabolic process; ketone body biosynthetic process; ketone body catabolic process Disease: Alpha-methylacetoacetic Aciduria |
| NCBI Summary: | This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009] |
| UniProt Code: | P24752 |
| NCBI GenInfo Identifier: | 135755 |
| NCBI Gene ID: | 38 |
| NCBI Accession: | P24752.1 |
| UniProt Secondary Accession: | P24752,Q96FG8, B2R6H1, G3XAB4, |
| UniProt Related Accession: | P24752 |
| Molecular Weight: | 17,175 Da |
| NCBI Full Name: | Acetyl-CoA acetyltransferase, mitochondrial |
| NCBI Synonym Full Names: | acetyl-CoA acetyltransferase 1 |
| NCBI Official Symbol: | ACAT1 |
| NCBI Official Synonym Symbols: | T2; MAT; ACAT; THIL |
| NCBI Protein Information: | acetyl-CoA acetyltransferase, mitochondrial |
| UniProt Protein Name: | Acetyl-CoA acetyltransferase, mitochondrial |
| UniProt Synonym Protein Names: | Acetoacetyl-CoA thiolase; T2 |
| UniProt Gene Name: | ACAT1 |
| UniProt Entry Name: | THIL_HUMAN |
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