ABI-1,E3B1,NAP1BP,SSH3BP1,Spectrin SH3 Domain Binding Protein 1,Abelson interactor 1,Abl-binding protein 4,Nap1-binding protein,Eps8 SH3 domain-binding protein
The Human ABL Interactor 1 (ABI1) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of ABI1 levels in human samples such as serum, plasma, and cell culture supernatants. ABI1 is a key protein that plays a vital role in cell signaling pathways, cell adhesion, and cytoskeletal organization.This ELISA kit provides researchers with a reliable and reproducible tool for studying the function of ABI1 in various biological processes.
ABI1 dysregulation has been implicated in diseases such as cancer, developmental disorders, and neurological conditions, making it a valuable biomarker for investigating disease mechanisms and potential therapeutic interventions.With its ease of use and high performance, the Human ABI1 ELISA Kit is an essential tool for researchers looking to advance their understanding of ABI1 biology and its implications in human health and disease.
Product Name:
Human Abl Interactor 1 (ABI1) ELISA Kit
Product Code:
HUDL00031
Size:
96 Assays
Target:
ABI1
Synonyms:
ABI-1, E3B1, NAP1BP, SSH3BP1, Spectrin SH3 Domain Binding Protein 1, Abelson interactor 1, Abl-binding protein 4, Nap1-binding protein, Eps8 SH3 domain-binding protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
