The Human Acylated Ghrelin ELISA Kit is a cutting-edge tool for the precise measurement of acylated ghrelin levels in human biological samples such as serum and plasma. With its superior sensitivity and specificity, this kit delivers dependable and consistent results, making it the perfect solution for various research needs.Acylated ghrelin is a pivotal hormone known for its role in regulating appetite and energy metabolism. Its dysregulation has been linked to various metabolic disorders, making it a valuable biomarker for studying conditions like obesity, diabetes, and metabolic syndrome.
By accurately quantifying acylated ghrelin levels, researchers can gain insights into the mechanisms underlying these conditions and explore potential therapeutic interventions.Overall, the Human Acylated Ghrelin ELISA Kit offers a reliable and convenient method for studying the physiological and pathological roles of acylated ghrelin, paving the way for advancements in metabolic research and potential therapeutic strategies.
Product Name:
Human A-GHRL (Acylated Ghrelin) ELISA Kit
SKU:
HUES02935
Target:
Human A-GHRL (Acylated Ghrelin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human A-GHRL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human A-GHRL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human A-GHRL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human A-GHRL. You can calculate the concentration of Human A-GHRL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
95-110
97-111
Average (%)
98
102
105
1:4
Range (%)
90-105
81-94
85-95
Average (%)
97
86
90
1:8
Range (%)
86-100
81-91
83-98
Average (%)
92
86
90
1:16
Range (%)
93-107
80-92
85-99
Average (%)
99
86
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-99
90
EDTA plasma (n=5)
86-100
93
Cell culture media (n=5)
86-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.89
139.44
441.98
59.26
140.77
399.38
Standard deviation
3.56
7.86
22.45
3.2
6.53
20.77
C V (%)
6.61
5.64
5.08
5.4
4.64
5.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human A-GHRL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human A-GHRL in samples. No significant cross-reactivity or interference between Human A-GHRL and analogues was observed.
