Human A Disintegrin And Metalloproteinase With Thrombospondin 9 (ADAMTS9) ELISA Kit
The Human ADAMTS9 (A Disintegrin and Metalloproteinase with Thrombospondin-9) ELISA Kit is specifically designed for the sensitive detection of ADAMTS9 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high accuracy and precision, ensuring consistent and reliable results for a variety of research applications.ADAMTS9 is a key enzyme involved in extracellular matrix remodeling and has been implicated in various diseases including cancer, inflammatory conditions, and cardiovascular disorders.
By measuring ADAMTS9 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Human ADAMTS9 ELISA Kit is an essential tool for studying the role of ADAMTS9 in disease pathology and development, providing valuable information for future research and drug discovery efforts.
Product Name:
Human A Disintegrin And Metalloproteinase With Thrombospondin 9 (ADAMTS9) ELISA Kit
Product Code:
HUDL00071
Size:
96 Assays
Target:
ADAMTS9
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
