Human A Disintegrin And Metalloproteinase With Thrombospondin 6 (ADAMTS6) ELISA Kit
The Human ADAMTS6 (A Disintegrin and Metalloproteinase with Thrombospondin-6) ELISA Kit is specifically designed for the accurate measurement of ADAMTS6 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures reliable and reproducible results, making it an excellent tool for various research applications.ADAMTS6 is a key protein involved in regulating the extracellular matrix and plays a crucial role in processes such as tissue remodeling, inflammation, and wound healing.
Dysregulation of ADAMTS6 has been linked to various diseases, including arthritis, cancer, and cardiovascular disorders, highlighting its significance as a potential biomarker for disease progression and therapeutic development.Overall, the Human ADAMTS6 ELISA Kit provides researchers with a powerful tool to study the role of ADAMTS6 in health and disease, offering valuable insights into potential therapeutic targets and diagnostic possibilities.
Product Name:
Human A Disintegrin And Metalloproteinase With Thrombospondin 6 (ADAMTS6) ELISA Kit
Product Code:
HUDL00069
Size:
96 Assays
Target:
ADAMTS6
Synonyms:
ADAM-TS6
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
