Human A Disintegrin And Metalloprotease 19 (ADAM19) ELISA Kit
SKU:
HUDL00060
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9H013
Sensitivity:
0.108ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
FKSG34,MADDAM,MLTNB,meltrin beta,Metalloprotease and disintegrin dendritic antigen marker,Disintegrin and metalloproteinase domain-containing protein 19
Human A Disintegrin And Metalloprotease 19 (ADAM19) ELISA Kit
The Human A Disintegrin and Metalloprotease 19 (ADAM19) ELISA Kit is a cutting-edge tool for accurate detection of ADAM19 levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.ADAM19 is a critical enzyme involved in cell signaling and tissue development, making it a key player in processes such as cell migration, adhesion, and proliferation. Dysregulation of ADAM19 has been linked to various diseases, including cancer, inflammatory disorders, and cardiovascular conditions.
As such, this ELISA kit serves as a valuable resource for studying these pathological conditions and exploring potential therapeutic interventions.With its advanced technology and precision, the Human ADAM19 ELISA Kit is a must-have for researchers and healthcare professionals seeking to deepen their understanding of ADAM19 biology and its implications in disease mechanisms. Trust in this kit to deliver reliable data and accelerate advancements in biomedical research.
Product Name:
Human A Disintegrin And Metalloprotease 19 (ADAM19) ELISA Kit
Product Code:
HUDL00060
Size:
96 Assays
Target:
ADAM19
Synonyms:
FKSG34, MADDAM, MLTNB, meltrin beta, Metalloprotease and disintegrin dendritic antigen marker, Disintegrin and metalloproteinase domain-containing protein 19
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
