Human A Disintegrin And Metalloprotease 12 (ADAM12) ELISA Kit
The Human ADAM12 ELISA Kit is specifically designed for the precise measurement of ADAM12 levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable tool for various research endeavors.ADAM12, a member of the ADAM (A Disintegrin And Metalloprotease) family, plays a critical role in processes such as cell adhesion, migration, and proteolysis.
Dysregulation of ADAM12 has been implicated in various diseases, including cancer, arthritis, and cardiovascular disorders, highlighting its importance as a potential biomarker for disease detection and therapy development.Overall, the Human ADAM12 ELISA Kit offers researchers a reliable and efficient way to study the role of ADAM12 in health and disease, paving the way for advancements in biomedical research and clinical diagnostics.
Product Name:
Human A Disintegrin And Metalloprotease 12 (ADAM12) ELISA Kit
Product Code:
HUDL00057
Size:
96 Assays
Target:
ADAM12
Synonyms:
MCMP, MCMPMltna, MLTN, MLTNA, Meltrin Alpha, Disintegrin and metalloproteinase domain-containing protein 12
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
