Human A beta1-42 (Amyloid Beta Peptide 1-42) ELISA Kit
The Human Aβ1-42 (Amyloid Beta Peptide 1-42) ELISA Kit is a powerful tool for accurate measurement of Aβ1-42 levels in human samples including serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures precise and reproducible results, making it ideal for a variety of research applications.Aβ1-42 is a key player in the pathogenesis of Alzheimer's disease, making it a critical biomarker for studying this condition and exploring potential therapeutic interventions.
By measuring Aβ1-42 levels, researchers can gain valuable insights into disease progression and potentially identify new targets for drug development.Overall, the Human Aβ1-42 (Amyloid Beta Peptide 1-42) ELISA Kit is a reliable tool for researchers looking to investigate the role of Aβ1-42 in neurodegenerative disorders and explore new avenues for treatment.
Product Name:
Human A beta1-42 (Amyloid Beta Peptide 1-42) ELISA Kit
SKU:
HUES01734
Target:
Human A beta1-42 (Amyloid Beta Peptide 1-42)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Aβ1-42. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Aβ1-42 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Aβ1-42, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Aβ1-42. You can calculate the concentration of Human Aβ1-42 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
94-110
86-98
Average (%)
95
100
91
1:4
Range (%)
98-118
87-99
95-107
Average (%)
107
93
100
1:8
Range (%)
95-109
87-101
94-104
Average (%)
101
94
99
1:16
Range (%)
94-108
85-101
98-114
Average (%)
100
92
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
93
EDTA plasma (n=5)
90-103
95
Cell culture media (n=5)
85-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.2
91.8
423.3
46.7
85.7
451.4
Standard deviation
2.8
4.8
21.2
2.9
4.4
14.9
C V (%)
5.69
5.23
5.01
6.21
5.13
3.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Aβ1-42 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Aβ1-42 in samples. No significant cross-reactivity or interference between Human Aβ1-42 and analogues was observed.
