Human 4BBL/TNFSF9 Recombinant Protein (RPES1680)

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SKU:
RPES1680
Product type:
Recombinant Protein
Host species:
E.coli
Reactivity:
Human
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Description

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Human 4BBL/TNFSF9 Recombinant Protein

Tumor necrosis factor ligand superfamily member 9(4-1BBL) is single-pass type II membrane protein which is a member of the the tumor necrosis factor family. 4-1BBL is a 254 amino acids cytokine that is expressed in brain; placenta; lung; skeletal muscle and kidney. TNFSF9 has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine may have a role in activation-induced cell death (AICD) and cognate interactions between T-cells and B-cells/macrophages. It has also been shown to be required for the optimal CD8 responses in CD8 T cells; and is thought to be involved in T cell-tumor cell interaction.

Product Name:Human 4BBL/TNFSF9 Recombinant Protein (RPES1680)
Product Code:RPES1680
Size:10µg
Species:Human
Expressed Host:E.coli
Synonyms:Tumor necrosis factor ligand superfamily member 9, 4-1BB ligand, 4-1BBL, TNFSF9
Accession:P41273
Sequence:Arg71-Glu254
Fusion tag:C-6His
Activity:Immobilized Human 4-1BB-His(Cat: PKSH032025) at 10µg/ml(100 ?l/well) can bind Biotinylated Human 4-1BBL-His. The ED50 of Human 4-1BBL-His is 147.3 ug/ml .
Endotoxin:<1.0 EU per µg as determined by the LAL method.
Protein Construction:Recombinant Human 4-1BB ligand is produced by our E.coli expression system and the target gene encoding Arg71-Glu254 is expressed with a 6His tag at the C-terminus.
Purity:> 95 % as determined by reducing SDS-PAGE.
Mol Mass:41.8 kDa
AP Mol Mass:16-20 kDa
Formulation:Lyophilized from a 0.2 µm filtered solution of 20mM PB;150mM NaCl;pH7.4.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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