Hemoglobin Assay Kit (Colorimetric) (BA0043)
- SKU:
- BA0043
- Product Type:
- Assay
- Detection Method:
- Colorimetric
- Instrument:
- Microplate Reader
- Sample Type:
- Blood, Serum, Plasma, Urine
- Research Area:
- Clinical Chemistry
Frequently bought together:
Description
Hemoglobin Assay Kit - Information
Assay Genie's hemoglobin assay kit is based on an improved Triton/NaOH method, in which the hemoglobin is converted into a uniform colored end product. The intensity of color, measured at 400 nm, is directly proportional to hemoglobin concentration in the sample. The optimized formulation exhibits high sensitivity and is ideal for measuring hemolysis in low hemoglobin samples (e.g. serum and plasma).Applications
For quantitative determination of hemoglobin and evaluation of drug effects on hemoglobin metabolism.Hemoglobin Assay Kit - Key Features
- Sensitive and accurate. Linear detection range 0.9 - 200 mg /dL hemoglobin in 96-well plate assay.
- Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.
- Safety. Reagents are non-toxic.
- Versatility. Assays can be executed in 96-well plate or cuvet.
Hemoglobin Assay Kit -Data Sheet | |
| Kit Includes | HB Reagent: 50 mL Calibrator: 10 mL |
| Kit Requires | Pipetting devices and accessories. |
| Method of Detection | OD400nm |
| Detection Limit | 0.9 mg/dL |
| Samples | Blood, serum, plasma, urine etc |
| Species | All |
| Protocol Length | 5 min |
| Size | 250 tests |
| Storage | Store reagent and calibrator at 4°C |
| Shelf Life | 12 months |
More Details
HEMOGLOBIN (Hb) is made of four globin chains each carrying a heme group. It is carried by red blood cells and transports oxygen from the lungs to the peripheral tissues to maintain the viability of cells. Quantitation of blood hemoglobin has been a key diagnostic parameter for various diseases such as anemia, polycythemia and dehydration. Simple, direct and automation-ready procedures for measuring hemoglobin concentration are becoming popular in Research and Drug Discovery.Related Products
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