Guinea pig Eosinophil Chemotactic Factor (ECF) ELISA Kit

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SKU:
GPDL00007
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
G8XRI2
Sensitivity:
6.2pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CCL11,SCYA11,Eotaxin 1,Eosinophil Chemotactic Protein 1,Chemokine C-C-Motif Ligand 11,Small Inducible Cytokine Subfamily A(Cys-Cys)Member 11
Reactivity:
Guinea Pig
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Frequently bought together:

Description

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Guinea pig Eosinophil Chemotactic Factor (ECF) ELISA Kit

The Guinea Pig Eosinophil Chemotactic Factor (ECF) ELISA Kit is specifically designed for the accurate detection of ECF levels in guinea pig serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and reproducible results for a variety of research applications.ECF is a vital factor in the recruitment and activation of eosinophils, a type of white blood cell involved in inflammation and allergic responses. It plays a crucial role in conditions such as asthma, allergic rhinitis, and certain skin disorders, making it an important biomarker for studying these diseases and developing potential treatments.

With easy-to-follow instructions and all necessary components included, the Guinea Pig Eosinophil Chemotactic Factor (ECF) ELISA Kit is a valuable tool for researchers looking to investigate the role of ECF in immune responses and inflammatory conditions in guinea pigs.

Product Name:Guinea pig Eosinophil Chemotactic Factor (ECF) ELISA Kit
Product Code:GPDL00007
Size:96 Assays
Target:ECF
Synonyms:Bovine Eosinophil Chemotactic Factor (ECF) ELISA Kit ECF DL-ECF-b CCL11 SCYA11 Eotaxin 1 Eosinophil Chemotactic Protein 1 Chemokine C-C-Motif Ligand 11 Small Inducible Cytokine Subfamily A(Cys-Cys)Member 11
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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