GST Activity Assay Kit (Colorimetric) (BA0028)

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SKU:
BA0028
Product Type:
Assay
Detection Method:
Colorimetric
Instrument:
Microplate Reader
Sample Type:
Biological Samples (Cell Lysates, Tissues)
Research Area:
Metabolism
Frequently bought together:

Description

ELISA Kit Technical ManualMSDS

GST Activity Assay Kit - Information

Assay Genie's Glutathione S-transferase, GST activity assay kit (colorimetric) is based on the GST enzyme reaction between GSH and the GST substrate, CDNB (1-chloro-2,4-dinitrobenzene). The GST catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected spectrophotometrically at 340 nm. The rate of increase in absorbance at 340 nm is directly proportional to the GST activity in the sample.

Applications

Glutathione S-transferase activity determination in biological samples

GST Activity Assay Kit - Key Features

  • Fast and sensitive. Linear detection range (20 uL sample): 2 to 80 U/L for 10 min reaction at 25°C.
  • Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

GST Activity Assay Kit - Data Sheet

Kit IncludesAssay Buffer: 25 mL Glutathione: Powder CDNB: 120 uL
Kit RequiresPipetting devices, centrifuge tubes, clear flat-bottom 96-well plates, and plate reader.
Method of DetectionOD340nm
Detection Limit2 U/L
SamplesBiological samples (e.g. cell lysates, tissues, etc.)
SpeciesAll
Protocol Length10 min
Size100 tests
Shelf Life6 months
StorageStore all components at 4°C upon receiving

More Details

GLUTATHIONE TRANSFERASE (GST; EC 2.5.1.18) is a multifunctionalenzyme that plays an important role in cellular detoxification. GST protects cells against foreign compounds such as carcinogens and drugs by catalyzing the attachment of glutathione to the compounds electrophilic and/or hydrophobic sites.

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