The GNRH (Gonadotropin-Releasing Hormone) ELISA Kit is a powerful tool for the quantitative measurement of GNRH levels in various biological samples, including serum, plasma, and tissue culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a wide range of research applications.GNRH is a critical hormone involved in the regulation of reproductive processes, including the release of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary gland. Dysregulation of GNRH signaling has been implicated in disorders such as infertility, polycystic ovarian syndrome, and hypogonadism, making it a key biomarker for understanding and diagnosing these conditions.
By accurately measuring GNRH levels, researchers can gain valuable insights into the mechanisms underlying reproductive health and disease, paving the way for new diagnostic tools and therapeutic strategies. With its high sensitivity and reliability, the GNRH ELISA Kit is an indispensable tool for investigating the role of GNRH in physiological and pathological processes.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with GnRH. During the reaction, GnRH in the sample or standard competes with a fixed amount of GnRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GnRH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GnRH in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
92-106
90-105
Average (%)
92
98
97
1:4
Range (%)
93-106
92-109
91-102
Average (%)
100
100
97
1:8
Range (%)
93-108
90-101
90-105
Average (%)
101
95
97
1:16
Range (%)
99-117
91-107
88-101
Average (%)
107
97
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-111
101
EDTA plasma (n=5)
88-100
95
Cell culture media (n=5)
87-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
281.2
937.8
2978.3
281.9
856.3
3080.8
Standard deviation
14.6
48.8
131.0
18.0
41.1
114.0
C V (%)
5.19
5.2
4.4
6.39
4.8
3.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of GnRH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes GnRH in samples. No significant cross-reactivity or interference between GnRH and analogues was observed.
