GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte macrophage progenitors. It is produced by a number of different cell types (including activated T cells; B cells; macrophages; mast cells; endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors; GM-CSF is also a growth factor for erythroid; megakaryocyte and eosinophil progenitors. On mature hematopoietic; monocytes/macrophages; and eosinophils; GM-CSF has also been reported to have a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally; GM-CSF can also stimulate the proliferation of a number of tumor cell lines; including osteogenic sarcoma; carcinoma and adenocarcinoma cell lines.
Product Name:
GM-CSF/CSF2 Recombinant Protein (P.pastoris) (RPES5183)
Measured in a cell proliferation assay using TF?1 human erythroleukemic cells. The ED50 for this effect is 6-30pg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor is produced by our Yeast expression system and the target gene encoding Ala18-Glu144 is expressed.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
14.4 kDa
AP Mol Mass:
24-35 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 10mM TrisHCl; 4% Mannitol; 1% Sucrose; pH 8.5.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
