Glucose Dehydrogenase Assay Kit (Colorimetric) (BA0024)

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SKU:
BA0024
Product Type:
Assay
Detection Method:
Colorimetric
Instrument:
Microplate Reader
Sample Type:
Biological Samples
Research Area:
Diabetes & Obesity
Plant & Environmental Stress
Clinical Chemistry
Frequently bought together:

Description

ELISA Kit Technical ManualMSDS

Glucose Dehydrogenase Assay Kit - Information

Assay Genie's non-radioactive, colorimetric GDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.

Applications

For quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism.

Glucose Dehydrogenase Assay Kit - Key Features

  • Fast and sensitive. Linear detection range (20 sample): 0.5 to 200 U/L for 15 min reaction.
  • Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Glucose Dehydrogenase Assay Kit - Data Sheet

Kit IncludesAssay Buffer: 10 mL Diaphorase: 120 uL NAD/MTT: 1 mL Calibrator: 1.5 mL Substrate: 1 mL
Kit RequiresPipetting devices and accessories (e.g. multi-channel pipettor), clear flat- bottom 96-well plates, centrifuge tubes and plate reader.
Method of DetectionOD565nm
Detection Limit0.5 U/L
SamplesBiological samples (e.g. plasma, serum, tissue and culture media).
SpeciesAll
Protocol Length15 min
Size100 tests
Shelf Life6 months
StorageStore all components at -20°C upon receiving

More Details

GLUCOSE DEHYDROGENASE (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donor with other acceptors. GDH participates in the pentose phosphate pathway.

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