The General Inositol Triphosphate (IP3) ELISA Kit is a highly reliable and sensitive tool for the accurate detection of IP3 levels in a variety of biological samples. This kit is designed for use with human serum, plasma, and cell culture supernatants, providing researchers with a versatile and effective solution for their IP3 measurement needs.IP3 is a key molecule involved in intracellular signaling, playing a critical role in various cellular processes such as cell growth, differentiation, and apoptosis. Dysregulation of IP3 signaling has been implicated in a range of diseases, including cancer, neurological disorders, and metabolic conditions. As such, the accurate measurement of IP3 levels is essential for understanding the underlying mechanisms of these diseases and developing potential therapeutic interventions.
With its high sensitivity and specificity, the General Inositol Triphosphate (IP3) ELISA Kit offers reliable and reproducible results, making it an invaluable tool for researchers working in the fields of cell biology, biochemistry, and molecular biology. Whether studying basic cellular signaling pathways or investigating disease mechanisms, this kit provides a robust platform for accurate IP3 quantification, enabling researchers to advance their understanding of intracellular signaling processes.
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
