The General Gibberellic Acid (GA) ELISA Kit is a highly reliable and accurate tool designed for the detection of Gibberellic Acid levels in various samples, including plant extracts, soil samples, and water. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for research applications in agriculture, environmental science, and plant biology.Gibberellic Acid is a key plant hormone that regulates various growth processes, including seed germination, stem elongation, and flowering.
By measuring Gibberellic Acid levels, researchers can gain valuable insights into plant growth and development, as well as study the effects of environmental factors on plant physiology.With its user-friendly protocol and robust performance, the General Gibberellic Acid (GA) ELISA Kit is an essential tool for researchers and scientists looking to explore the role of Gibberellic Acid in plant biology and environmental science.
Product Name:
General Gibberellic Acid (GA) ELISA Kit
Product Code:
UNDL00039
Size:
96 Assays
Target:
GA
Synonyms:
GA3, Gibberellin A3, Gibberellins
Detection Method:
Competition
Range:
156-10,000ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
