Based on the information provided in the URL, here is the product description for the General Gamma-Aminobutyric Acid (GABA) ELISA Kit:The General Gamma-Aminobutyric Acid (GABA) ELISA Kit is designed for the precise measurement of GABA levels in various samples including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and consistent results for a wide range of research applications.Gamma-aminobutyric acid (GABA) is a crucial neurotransmitter in the brain that plays a key role in regulating neuronal activity and promoting relaxation.
Imbalances in GABA levels have been implicated in various neurological conditions such as anxiety, epilepsy, and sleep disorders, making it a valuable biomarker for studying these disorders and developing potential treatments.Overall, the General Gamma-Aminobutyric Acid (GABA) ELISA Kit is a reliable tool for researchers looking to investigate the role of GABA in neurological function and disorders, providing valuable insights for potential therapeutic interventions.
Product Name:
General Gamma-Aminobutyric Acid (gABA) ELISA Kit
Product Code:
UNDL00040
Size:
96 Assays
Target:
gABA
Synonyms:
4-Aminobutyric Acid
Detection Method:
Competition
Range:
31.25-2,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
