The General Free Thyroxine (FT4) ELISA Kit from Assay Genie is a highly reliable and sensitive assay designed for the accurate detection of free thyroxine levels in human serum, plasma, and cell culture supernatants. This kit provides researchers with a powerful tool for studying thyroid function and evaluating thyroid hormone levels in various physiological and pathological conditions.Thyroxine, also known as T4, is an important thyroid hormone that regulates metabolism, growth, and development in the body. Monitoring free thyroxine levels is crucial for diagnosing thyroid disorders such as hypothyroidism and hyperthyroidism, as well as for managing thyroid hormone replacement therapy.
With its high sensitivity and specificity, the General Free Thyroxine (FT4) ELISA Kit offers researchers a reliable and reproducible method for quantifying free thyroxine levels in biological samples. This kit is ideal for a wide range of research applications, including clinical diagnostics, pharmaceutical development, and basic research in endocrinology and thyroid biology.Overall, the General Free Thyroxine (FT4) ELISA Kit from Assay Genie provides an essential tool for studying thyroid function and assessing free thyroxine levels in a variety of research settings. Trust in Assay Genie for accurate and reliable results in your thyroid research.
Product Name:
General Free Thyroxine (fT4) ELISA Kit
Product Code:
UNDL00038
Size:
96 Assays
Target:
fT4
Synonyms:
FTI
Detection Method:
Competition
Range:
1.406-90pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
