The General Arachidonic Acid (AA) ELISA Kit from Assay Genie is a powerful tool for accurately measuring levels of arachidonic acid in various biological samples, including serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, providing reliable and reproducible results for researchers in a wide range of fields.Arachidonic acid is a key fatty acid involved in inflammation and the production of important signaling molecules in the body. Monitoring arachidonic acid levels can provide valuable insights into inflammatory processes, cardiovascular health, and neurological disorders.
The General Arachidonic Acid (AA) ELISA Kit is essential for studying these conditions and developing potential therapeutic interventions.Don't miss out on the opportunity to advance your research with the General Arachidonic Acid (AA) ELISA Kit from Assay Genie. Order yours today and unlock new possibilities in your scientific endeavors.
Product Name:
General Arachidonic Acid (AA) ELISA Kit
Product Code:
UNDL00006
Size:
96 Assays
Target:
AA
Synonyms:
ARA
Detection Method:
Competition
Range:
4.687-300ug/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
