The General 5-Hydroxytryptamine (5-HT) ELISA Kit from AssayGenie is a valuable tool for researchers working with serotonin, a neurotransmitter known to play a key role in mood regulation, sleep, and appetite. This kit allows for the precise measurement of 5-HT levels in a variety of biological samples, including serum, plasma, and tissue homogenates.With its high sensitivity and specificity, the General 5-HT ELISA Kit ensures accurate and reliable results, making it ideal for a wide range of research applications.
By studying 5-HT levels, researchers can gain insights into various physiological and pathological processes, including depression, anxiety, and gastrointestinal disorders.Don't miss out on the opportunity to deepen your understanding of serotonin and its impact on human health with the General 5-Hydroxytryptamine (5-HT) ELISA Kit from AssayGenie.
Product Name:
General 5-Hydroxytryptamine (5-HT) ELISA Kit
Product Code:
UNDL00003
Size:
96 Assays
Target:
5-HT
Synonyms:
5HT, Serotonin, N-Methyl-Gamma
Detection Method:
Competition
Range:
15.625-1000ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
Yu et al.
Network pharmacology and experimental verification to explore the anti-migraine mechanism of Yufeng Ningxin Tablet
