FOLR2 Antibody (PACO58761)
- SKU:
- PACO58761
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | FOLR2 Antibody (PACO58761) |
| Antibody SKU: | PACO58761 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Folate receptor β protein (35-167AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO58761 diluted at 1:400 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO58761 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Binds to folate and reduced folic acid, derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid, analogs at neutral pH. Exposure to slightly acid, c pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release. |
| Synonyms: | Folate receptor β (FR-beta) (Folate receptor 2) (Folate receptor, fetal/placental) (Placental folate-binding protein) (FBP), FOLR2 |
| UniProt Protein Function: | FOLR2: Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. Belongs to the folate receptor family. |
| UniProt Protein Details: | Protein type:Membrane protein, GPI anchor Chromosomal Location of Human Ortholog: 11q13.3-q13.5 Cellular Component: anchored to external side of plasma membrane; cell surface; membrane Molecular Function:drug binding; folic acid binding; folic acid transporter activity; methotrexate binding Biological Process: folic acid transport; monocyte chemotaxis; positive regulation of cell proliferation; receptor-mediated endocytosis; regulation of thymidylate synthase biosynthetic process |
| NCBI Summary: | The protein encoded by this gene is a member of the folate receptor (FOLR) family, and these genes exist in a cluster on chromosome 11. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and they mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This protein has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. Although this protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients. Multiple transcript variants that encode the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P14207 |
| NCBI GenInfo Identifier: | 116241366 |
| NCBI Gene ID: | 2350 |
| NCBI Accession: | P14207.4 |
| UniProt Secondary Accession: | P14207,Q05CA5, Q6GTE8, |
| UniProt Related Accession: | P14207 |
| Molecular Weight: | 29,280 Da |
| NCBI Full Name: | Folate receptor beta |
| NCBI Synonym Full Names: | folate receptor beta |
| NCBI Official Symbol: | FOLR2 |
| NCBI Official Synonym Symbols: | FBP; FR-P3; FR-BETA; BETA-HFR; FBP/PL-1 |
| NCBI Protein Information: | folate receptor beta |
| UniProt Protein Name: | Folate receptor beta |
| UniProt Synonym Protein Names: | Folate receptor 2; Folate receptor, fetal/placental; Placental folate-binding protein; FBP |
| Protein Family: | Folate receptor |
| UniProt Gene Name: | FOLR2 |
| UniProt Entry Name: | FOLR2_HUMAN |
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