Equine IL 2 Recombinant Protein (RPPB0485)
- SKU:
- RPPB0485
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Equine
- Target:
- IL 2
- Synonyms:
- Interleukin-2
- IL-2
- T-cell growth factor
- TCGF
- Source:
- Escherichia Coli
- Uniprot:
- P37997
Description
| Product Name: | Equine IL 2 Recombinant Protein |
| Product Code: | RPPB0485 |
| Size: | 20µg |
| Species: | Equine |
| Target: | IL 2 |
| Synonyms: | Interleukin-2, IL-2, T-cell growth factor, TCGF, IL2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 6.0. |
| Solubility: | It is recommended to reconstitute the lyophilized IL2 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Interleukin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by SDS-PAGE and HPLC analyses. |
| Amino Acid Sequence: | APTSSSKRET QQQLKQLQMD LKLLLEGVNN NKNPKLSKML TFKINMPKKA TELKHLQCLE EELKPLEEML KNFLSKDIKE LMSNINVTVL GLKGSETRFT CEYDDETGTI VEFLNKWITF SQSIFSTMT |
| Biological Activity: | The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 1.0 µg/ml, corresponding to a specific activity of > 1000 IU/mg. |
| UniProt Code: | P37997 |
IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
Recombinant Equine Interleukin-2 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 129 amino acids (with a substitution of S for C - at position 141 compared with the wild type IL2) and having a molecular mass of 14.9kDa.The IL-2 Equine is purified by proprietary chromatographic techniques.
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