Elk1 (Phospho-Ser383) Transcription Factor Activity Assay
- SKU:
- TFAB00021
- Product Type:
- ELISA Kit
- ELISA Type:
- Transcription Factor Assay
- Analytes:
- Elk1 (Phospho-Ser383)
- Reactivity:
- Human
- Mouse
Description
Elk1 (Phospho-Ser383)Transcription Factor Activity Assay
The ELK1 Phospho-Ser383 Transcription Factor Activity Assay Kit is a powerful tool for studying the activity of the ELK1 transcription factor in cell signaling pathways. This kit is designed for the accurate and reliable detection of ELK1 phosphorylation at Ser383 in cell lysates, providing valuable insights into the regulation of gene expression and cellular processes.ELK1 is a key transcription factor that plays a critical role in cell growth, differentiation, and survival. Phosphorylation of ELK1 at Ser383 is known to enhance its transcriptional activity, making this modification a crucial event in signal transduction pathways. By measuring the levels of phosphorylated ELK1, researchers can gain a better understanding of how cells respond to external stimuli and how gene expression is regulated in various physiological and pathological conditions.
With its high sensitivity and specificity, the ELK1 Phospho-Ser383 Transcription Factor Activity Assay Kit offers researchers a reliable and reproducible method for studying ELK1 signaling pathways. Whether investigating the molecular mechanisms underlying diseases such as cancer, inflammation, or neurodegenerative disorders, this kit provides a valuable tool for advancing research in the field of cell biology and molecular medicine.
| Product Name: | Elk1 (Phospho-Ser383) Transcription Factor Activity Assay |
| Product Code: | TFAB00021 |
| Target: | Elk1 (Phospho-Ser383) |
| Reactivity: | Human, Mouse |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie Elk1 (Phospho-Ser383) transcription factor activity assay allows for the detection and qualitative analysis of Elk1 (Phospho-Ser383) active transcription factors in eukaryotic nuclear or cell lysates.
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | ELK1: a transcription factor of the Ets family and the ternary complex factor (TCF) subfamily. Forms a ternary complex by binding to the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. Mediates gene activity in response to serum and growth factors. Phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C-terminus; phosphorylation at these sites is critical for transcriptional activation by Elk-1. Two alternatively-spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: Xp11.2 Cellular Component: cell soma; dendrite; mitochondrion; nerve terminal; nucleoplasm; nucleus Molecular Function:chromatin binding; protein binding; transcription factor activity Biological Process: cell differentiation; innate immune response; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; response to light stimulus; stress-activated MAPK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; transcription from RNA polymerase II promoter |
| NCBI Summary: | This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012] |
| UniProt Code: | P19419 |
| NCBI GenInfo Identifier: | 12643407 |
| NCBI Gene ID: | 2002 |
| NCBI Accession: | P19419.2 |
| UniProt Secondary Accession: | P19419,O75606, O95058, Q969X8, Q9UJM4, B2R7H4, |
| UniProt Related Accession: | P19419 |
| Molecular Weight: | |
| NCBI Full Name: | ETS domain-containing protein Elk-1 |
| NCBI Synonym Full Names: | ELK1, ETS transcription factor |
| NCBI Official Symbol: | ELK1 |
| NCBI Protein Information: | ETS domain-containing protein Elk-1 |
| UniProt Protein Name: | ETS domain-containing protein Elk-1 |
| Protein Family: | ETS domain-containing protein |
| UniProt Gene Name: | ELK1 |
| UniProt Entry Name: | ELK1_HUMAN |
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