DUSP4 Antibody (PACO62463)
- SKU:
- PACO62463
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | DUSP4 Antibody (PACO62463) |
| Antibody SKU: | PACO62463 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:200-1:500 |
| Species Reactivity: | Human, Mouse |
| Immunogen: | Recombinant Human Dual specificity protein phosphatase 4 protein (109-198AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: HepG2 whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate. All lanes: DUSP4 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 43, 33 kDa. Observed band size: 33 kDa. |
 | IHC image of PACO62463 diluted at 1:200 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Regulates mitogenic signal transduction by dephosphorylating both Thr and Tyr residues on MAP kinases ERK1 and ERK2. |
| Synonyms: | Dual specificity protein phosphatase 4 (EC 3.1.3.16) (EC 3.1.3.48) (Dual specificity protein phosphatase hVH2) (Mitogen-activated protein kinase phosphatase 2) (MAP kinase phosphatase 2) (MKP-2), DUSP4, MKP2 VH2 |
| UniProt Protein Function: | MKP-2: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP4 inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Induced by hepatocyte growth factor (HGF) and vascular endothelial cell growth factor (VEGF) in human endothelial cells. Two alternatively spliced isoforms have been described. In addition, multiple polyadenylation sites have been reported. Contains 1 rhodanese domain. |
| UniProt Protein Details: | Protein type:Protein phosphatase, dual-specificity; EC 3.1.3.48; EC 3.1.3.16 Chromosomal Location of Human Ortholog: 8p12-p11 Cellular Component: nucleoplasm; nucleus Molecular Function:MAP kinase tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; protein tyrosine/threonine phosphatase activity Biological Process: endoderm formation; inactivation of MAPK activity; innate immune response; MAPKKK cascade; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; nerve growth factor receptor signaling pathway; protein amino acid dephosphorylation; stress-activated MAPK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway |
| NCBI Summary: | The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Two alternatively spliced transcript variants, encoding distinct isoforms, have been observed for this gene. In addition, multiple polyadenylation sites have been reported. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13115 |
| NCBI GenInfo Identifier: | 2499745 |
| NCBI Gene ID: | 1846 |
| NCBI Accession: | Q13115.1 |
| UniProt Secondary Accession: | Q13115,Q13524, B2RBU5, D3DSU4, G5E930, |
| UniProt Related Accession: | Q13115 |
| Molecular Weight: | 32,993 Da |
| NCBI Full Name: | Dual specificity protein phosphatase 4 |
| NCBI Synonym Full Names: | dual specificity phosphatase 4 |
| NCBI Official Symbol: | DUSP4 |
| NCBI Official Synonym Symbols: | TYP; HVH2; MKP2; MKP-2 |
| NCBI Protein Information: | dual specificity protein phosphatase 4 |
| UniProt Protein Name: | Dual specificity protein phosphatase 4 |
| UniProt Synonym Protein Names: | Dual specificity protein phosphatase hVH2; Mitogen-activated protein kinase phosphatase 2; MAP kinase phosphatase 2; MKP-2 |
| Protein Family: | Dual specificity protein phosphatase |
| UniProt Gene Name: | DUSP4 |
| UniProt Entry Name: | DUS4_HUMAN |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
Related Products
Human DUSP4 / MKP2 ELISA Kit
