DOHH Antibody (PACO60376)
- SKU:
- PACO60376
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | DOHH Antibody (PACO60376) |
| Antibody SKU: | PACO60376 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Deoxyhypusine hydroxylase protein (79-159AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunofluorescence staining of PC-3 cells with PACO60376 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor. |
| Synonyms: | Deoxyhypusine hydroxylase (hDOHH) (EC 1.14.99.29) (Deoxyhypusine dioxygenase) (Deoxyhypusine monooxygenase) (HEAT-like repeat-containing protein 1), DOHH, HLRC1 |
| UniProt Protein Function: | DOHH: Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)- L-lysine intermediate to form hypusine, an essential post- translational modification only found in mature eIF-5A factor. Belongs to the deoxyhypusine hydroxylase family.Protein type: Oxidoreductase; EC 1.14.99.29Chromosomal Location of Human Ortholog: 19p13.3Cellular Component: cytosolMolecular Function: protein binding; metal ion binding; deoxyhypusine monooxygenase activityBiological Process: cellular protein metabolic process; hypusine biosynthetic process from peptidyl-lysine; post-translational protein modification |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a metalloenzyme that catalyzes the last step in the conversion of lysine to the unique amino acid hypusine in eukaryotic initiation factor 5A. The encoded protein hydroxylates deoxyhypusine to form hypusine in the mature eukaryotic initiation factor 5A protein. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2009] |
| UniProt Code: | Q9BU89 |
| NCBI GenInfo Identifier: | 223633884 |
| NCBI Gene ID: | 83475 |
| NCBI Accession: | NP_001138637.1 |
| UniProt Secondary Accession: | Q9BU89,O75265 |
| UniProt Related Accession: | Q9BU89 |
| Molecular Weight: | 32,904 Da |
| NCBI Full Name: | deoxyhypusine hydroxylase |
| NCBI Synonym Full Names: | deoxyhypusine hydroxylase/monooxygenase |
| NCBI Official Symbol: | DOHH |
| NCBI Official Synonym Symbols: | HLRC1; hDOHH |
| NCBI Protein Information: | deoxyhypusine hydroxylase; deoxyhypusine dioxygenase; deoxyhypusine monooxygenase; HEAT-like repeat-containing protein 1; HEAT-like (PBS lyase) repeat containing 1 |
| UniProt Protein Name: | Deoxyhypusine hydroxylase |
| UniProt Synonym Protein Names: | Deoxyhypusine dioxygenase; Deoxyhypusine monooxygenase; HEAT-like repeat-containing protein 1 |
| Protein Family: | Deoxyhypusine hydroxylase |
| UniProt Gene Name: | DOHH |
| UniProt Entry Name: | DOHH_HUMAN |
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