DNA Polymerase Beta/POLB Recombinant Protein (RPES2462)
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- SKU:
- RPES2462
- Product type:
- Recombinant Protein
- Host species:
- E.coli
- Reactivity:
- Human
Frequently bought together:
Description
system_update_alt데이터시트
DNA Polymerase Beta/POLB Recombinant Protein
Human DNA polymerase β is constitutively expressed in cells. It fills in gaps in DNA that are formed following base excision repair. Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. It conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. The activity cannot be affected by Aphidicolin, which is an inhibitor of DNA polymerase β.
| Product Name: | DNA Polymerase Beta/POLB Recombinant Protein (RPES2462) |
| Product Code: | RPES2462 |
| Size: | 10µg |
| Species: | Human |
| Expressed Host: | E.coli |
| Synonyms: | DNA Polymerase Beta, POLB |
| Accession: | P06746 |
| Sequence: | Ser2-Glu335 |
| Fusion tag: | C-6His |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | Recombinant Human DNA Polymerase beta is produced by our E.coli expression system and the target gene encoding Ser2-Glu335 is expressed with a 6His tag at the C-terminus. |
| Purity: | > 95 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 39.2 kDa |
| AP Mol Mass: | 39 kDa |
| Formulation: | Supplied as a 0.2 µm filtered solution of 20mM TrisHCl, 100mM NaCl, 1mM DTT, 1mM EDTA, 50% Glycerol, pH 7.8. |
| Shipping: | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C. |
| Stability and Storage: | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
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