Anti-CYP8B1 항체의 (PACO56776)

Antibody Name:	CYP8B1 Antibody (PACO56776)
Antibody SKU:	PACO56776
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human, Rat
Immunogen:	Recombinant Human 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase protein (303-440AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: Rat kidney tissue. All lanes: CYP8B1 antibody at 6.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 59 kDa. Observed band size: 59 kDa.
	IHC image of PACO56776 diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
	IHC image of PACO56776 diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:	Involved in bile acid, synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid, and chenodeoxycholic acid, Has a rather broad substrate specificity including a number of 7-alpha-hydroxylated C27 steroids.
Synonyms:	7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase (EC 1.14.18.8) (7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase) (CYPVIIIB1) (Cytochrome P450 8B1) (Sterol 12-alpha-hydroxylase), CYP8B1, CYP12

UniProt Protein Function:	CYP8B1: Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha- hydroxylated C27 steroids. Belongs to the cytochrome P450 family.Protein type: Lipid Metabolism - primary bile acid biosynthesis; Membrane protein, integral; EC 1.14.13.95; OxidoreductaseChromosomal Location of Human Ortholog: 3p22.1Cellular Component: endoplasmic reticulum membraneMolecular Function: 7alpha-hydroxycholest-4-en-3-one 12alpha-hydroxylase activity; oxygen binding; sterol 12-alpha-hydroxylase activityBiological Process: bile acid biosynthetic process; sterol metabolic process
UniProt Protein Details:
NCBI Summary:	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UNU6
NCBI GenInfo Identifier:	308153428
NCBI Gene ID:	1582
NCBI Accession:	Q9UNU6.2
UniProt Secondary Accession:	Q9UNU6,O75958, Q6NWT2, Q6NWT3, B2RCY3
UniProt Related Accession:	Q9UNU6
Molecular Weight:	58,068 Da
NCBI Full Name:	7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase
NCBI Synonym Full Names:	cytochrome P450 family 8 subfamily B member 1
NCBI Official Symbol:	CYP8B1
NCBI Official Synonym Symbols:	CP8B; CYP12
NCBI Protein Information:	7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase
UniProt Protein Name:	7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase
UniProt Synonym Protein Names:	7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; CYPVIIIB1; Cytochrome P450 8B1; Sterol 12-alpha-hydroxylase
Protein Family:	7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase
UniProt Gene Name:	CYP8B1
UniProt Entry Name:	CP8B1_HUMAN

Antibodies

CYP8B1 Antibody (PACO00673)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)