Chicken Growth Hormone Recombinant Protein (RPPB0330)
- SKU:
- RPPB0330
- Product type:
- Recombinant Protein
- Size:
- 100ug
- Species:
- Chicken
- Target:
- Growth Hormone
- Synonyms:
- GH1
- GH
- GHN
- GH-N
- Source:
- Escherichia Coli
- Uniprot:
- P08998
Description
| Product Name: | Chicken Growth Hormone Recombinant Protein |
| Product Code: | RPPB0330 |
| Size: | 100µg |
| Species: | Chicken |
| Target: | Growth Hormone |
| Synonyms: | GH1, GH, GHN, GH-N, hGH-N,Pituitary GH, GH 1, |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The protein was lyophilized from a concentrated (1mg/ml) solution with 0.3% NaHCO3 adjusted to pH 8. |
| Solubility: | It is recommended to reconstitute the lyophilized GH Chicken Recombinant in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml and not more than 3 mg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein. |
| Stability: | Lyophilized GH Chicken although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution at > 0.1 mg/ml and filter sterilization GH can be stored at 4°C for several weeks. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is recommended.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 99.0% as determined by:(a) Analysis by SDS-PAGE gel.(b) Analysis by SEC-HPLC. |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Phe-Pro-Ala |
| Biological Activity: | GH Chicken Recombinantis fully biologically active in homologous assays and in PDF-P1 3B9 cells stably transfected with rabbit GH receptors. |
GH is a member of the prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the GH locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five GHs, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the GH locus. Mutations in or deletions of the gene lead to GH deficiency and short stature.
GH Chicken Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 191 amino acids with an additional Ala at its N-terminus and having a molecular mass of 22255 Dalton. GH Chicken recombinant is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Growth hormone plays an important role in growth control. |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | P08998 |
| NCBI GenInfo Identifier: | 46048939 |
| NCBI Gene ID: | 378781 |
| NCBI Accession: | NP_989690.1 |
| UniProt Secondary Accession: | P08998,Q53Z10 |
| UniProt Related Accession: | P08998 |
| Molecular Weight: | 24,713 Da |
| NCBI Full Name: | somatotropin |
| NCBI Synonym Full Names: | growth hormone |
| NCBI Official Symbol: | GH |
| NCBI Official Synonym Symbols: | GH1; cGH |
| NCBI Protein Information: | somatotropin |
| UniProt Protein Name: | Somatotropin |
| UniProt Synonym Protein Names: | Growth hormone |
| Protein Family: | Envelope glycoprotein |
| UniProt Gene Name: | GH |
| UniProt Entry Name: |
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