Cellular Reactive Oxygen Species (ROS) Assay Kit (CV0022)

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SKU:
CV0022
Product Type:
Assay
Research Area:
Metabolism
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Description

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Cellular Reactive Oxygen Species (ROS) Assay Kit

ROS Detection Assay Kit is a highly sensitive and safe assay to measure Reactive Oxygen Species. ROS include a number of reactive molecules and free radicals, derived from molecular oxygen, that damage DNA and RNA and oxidize proteins and lipids (lipid peroxidation). The most common ROS include superoxide anion (O2-), hydrogen peroxide (H2O2), hydroxyl radical (HO-) and singlet oxygen (1O2), all of which are more reactive than oxygen (O2) itself. The molecules are produced during the electron transport of mitochondrial aerobic respiration or by oxidoreductase enzymes and metal catalysed oxidation.

It uses the cell permeant reagent Dichlorodihydrofluorescein-diacetate (H2DCFDA), a fluorogenic dye that measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity within the cell. After diffusion in to the cell, the acetyl groups on H2DCFDA are cleaved by intracellular esterase to yield the non-fluorescent compound which is rapidly oxidized to highly fluorescent 2',7'-Dichlorodihydrofluorescein by ROS. The fluorescence intensity is proportional to the ROS levels within the cell cytosol.

Kit Information:

Overview Description

Size:

5×96 assays

Advantages & Features:
  • Fluorescent based assay: Ex/Em = 485/530 nm
  • Based on unique fluorescent probe H2DCFDA.
  • Fast & Easy Protocol.
  • Safe: non-Radioactive, no special handling needed.
  • High-Throughput.
  • Accurate & Reproducible results.

Kit Contents:
  • 5 mg Fluorescent Substrate (H2DCFDA)
  • 50 mL 10X Assay Buffer
  • 500 μL Positive control (H2O2, 8.8M)
  • 1 mL DMSO

Applications:
  • Measurement of intracellular levels of ROS
  • Microplate reader (Ex/Em = 485/530 nm)
  • Microscope with TRITC channel
  • Flow Cytometry: FL1 Channel

Shipping & Storage:
  • Shipment Gel Pack.
  • Storage: 4 °C upon receipt.
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Safety Information:

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Citations

Hwang et al.Reactive oxygen species-generating hydrogel platform for enhanced antibacterial therapyNPG Asia Materials 2022View Citation

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