Cell Viability Assay (Colorimetric/Fluorometric) (BA0003)

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SKU:
BA0003
Product Type:
Assay
Detection Method:
Colorimetric
Detection Method:
Fluorometric
Sample Type:
Cell Culture
Research Area:
Cell Viability & Proliferation
High-Throughput Screening
Frequently bought together:

Description

ELISA Kit Technical ManualMSDS

Cell Viability Assay - Information

Cell Viability Assay Kit (Colorimetric/Fluorometric) reagent has been optimized for maximum sensitivity, reproducibility and long shelf-life. The homogeneous cell-based assay can be performed in multi-well plates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates. Applications include cell proliferation, cytotoxicity and apoptosis.

Applications

Homogeneous assay for cell viability, proliferation, cytotoxcity and high-throughput screen for anticancer agents.

Cell Viability Assay - Key Features

  • Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay).
  • Sensitive and accurate. As low as 100 cells can be accurately quantified.
  • Time efficient. High-throughput assay in 96-well and 384-well plates allows simultaneous processing ten of thousands of samples per day.
  • Homogeneous and convenient. A single reagent and "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.
  • Robust and amenable to HTS. Z' factors of 0.6 to 0.9 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.

Cell Viability Assay - Data Sheet

IncludesCatalog # Size (assays) Reagent BA0002 5,000 50 mL BA0003 10,000 100 mL BA0004 500 10ml.Control Reagent (Cat # BA0005): 50 mg saponin (to be ordered separately).
Method of DetectionFL530/590nm
Detection Limit100 cells
SamplesCell culture
SpeciesAll
Protocol Length1-5 hrs
Size10000 tests
StorageThe Blue reagent is light sensitive and should be stored in the provided amber bottle at 4°C. The Control Reagent is stable at -20°C.
Shelf Life12 months

More Details

This homogeneous assay involves simply adding a single reagent, the colorimetric/fluorometric Cell Viability Assay Kit reagent, to the cell culture and measuring the fluorescence intensity (excitation wavelength = 530 - 570 nm, emission wavelength = 590 - 620 nm) after an incubation step.

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