Carbonic anhydrase 5B; also known as carbonate dehydratase VB; carbonic anhydrase VB; CA-VB and CA5B; is a member of the alpha-carbonic anhydrase family. The strongest expression of CA5B / CA-VB is in heart; pancreas; kidney; placenta; lung; and skeletal muscle. It is not expressed in liver. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes; including respiration; calcification; acid-base balance; bone resorption; and the formation of aqueous humor; cerebrospinal fluid; saliva; and gastric acid. CAs show extensive diversity in tissue distribution and in their subcellular localization. CA5B / CA-VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA5A / CA-VA. CA5B / CA-VB has a wider tissue distribution than CA5A / CA-VA; which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles. CA5A / CA-VA is activated by histamine; L-adrenaline; L- and D-histidine; and L- and D-phenylalanine. It is inhibited by coumarins; sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt). CA5B / CA-VB is inhibited by coumarins; sulfonamide derivatives such as acetazolamide (AZA); saccharin and Foscarnet (phosphonoformate trisodium salt).
Product Name:
Carbonic Anhydrase 5B/CA5B Recombinant Protein (RPES5221)
Measured by its esterase activity. The specific activity is >150 pmoles/min/µg.
Endotoxin:
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Protein Construction:
A DNA sequence encoding the mature form of human CA5B (Q9Y2D0) (Cys 34-Pro 317) was fused with a polyhistidine tag at the C-terminus and an initial Met at the N-terminus.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
