Canine IFNG Recombinant Protein (RPPB0404)

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SKU:
RPPB0404
Product type:
Recombinant Protein
Size:
20ug
Species:
Canine
Target:
IFNG
Synonyms:
Immune Interferon
Synonyms:
type II interferon
Synonyms:
T cell interferon
Synonyms:
MAF
Source:
Escherichia Coli
Uniprot:
P42161
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Description

Product Name:Canine IFNG Recombinant Protein
Product Code:RPPB0404
Size:20µg
Species:Canine
Target:IFNG
Synonyms:Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:The IFNG was lyophilized from a 0.2µm filtered concentrated solution in 25mM Sodium Succinate, pH 5.0, 60mM NaCl, with 0.1% Tween-80.
Solubility:It is recommended to reconstitute the lyophilized IFNG in sterile distilled H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized IFNG although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFNG should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:QAMFFKEIEN LKEYFNASNP DVSDGGSLFV DILKKWREES DKTIIQSQIV SFYLKLFDNF KDNQIIQRSM DTIKEDMLGK FLNSSTSKRE DFLKLIQIPV NDLQVQRKAI NELIKVMNDL SPRSNLRKRK RSQNLFRGRR ASK
Biological Activity:The ED50 as determined by an anti-viral assay using A-72 canine fibroma cells infected with vesicular stomatitis virus (VSV) is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.

IFN-gamma produced by lymphocytes activated by specific antigens or mitogens.IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Recombinant IFNG Dog produced in E.coli cells is a non-glycosylated, homodimeric protein containing 143 amino acid chain and having a molecular mass of 16.9kDa. IFNG is purified by proprietary chromatographic techniques.

UniProt Protein Function:Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
UniProt Code:P42161
NCBI GenInfo Identifier:12644013
NCBI Gene ID:403801
NCBI Accession:P42161.2
UniProt Secondary Accession:P42161,Q9TVA0, A1E294,
UniProt Related Accession:P42161
Molecular Weight:19,395 Da
NCBI Full Name:Interferon gamma
NCBI Synonym Full Names:interferon gamma
NCBI Official Symbol:IFNG  
NCBI Official Synonym Symbols:IFN-G; IFN-gamma  
NCBI Protein Information:interferon gamma
UniProt Protein Name:Interferon gamma
Protein Family:Interferon
UniProt Gene Name:IFNG  
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