Canine Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) ELISA Kit

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SKU:
CNDL00031
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P48749
Sensitivity:
7.2pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CSF2,GM-CSF,Sargramostim,Molgramostin,Granulocyte-Macrophage Colony Stimulating Factor
Reactivity:
Canine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Frequently bought together:

Description

system_update_altDatasheet

Canine Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) ELISA Kit

The Canine GM-CSF (Granulocyte Macrophage Colony-Stimulating Factor) ELISA Kit is a powerful tool for accurately measuring GM-CSF levels in canine samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring precise and reproducible results for various research applications.GM-CSF is a key cytokine that regulates the production and function of granulocytes and macrophages, critical components of the immune system.

Its dysregulation has been linked to inflammatory and autoimmune diseases in canines, making it a valuable biomarker for studying these conditions and developing therapeutic interventions.With the Canine GM-CSF ELISA Kit, researchers can gain valuable insights into the immune response and inflammatory processes in canine diseases, ultimately advancing our understanding and treatment of these conditions.

Product Name:Canine Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) ELISA Kit
Product Code:CNDL00031
Size:96 Assays
Target:GMCSF
Synonyms:CSF2, GM-CSF, Sargramostim, Molgramostin, Granulocyte-Macrophage Colony Stimulating Factor
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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