The Canine Collagen Type I Alpha 2 (COL1A2) ELISA Kit is a powerful tool for the accurate measurement of collagen levels in canine serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reliable results, making it ideal for a wide range of research applications in the field of veterinary medicine.Collagen Type I Alpha 2 is a key component of the extracellular matrix and plays a crucial role in maintaining tissue structure and function. Abnormalities in collagen levels have been linked to various diseases and conditions in dogs, making this ELISA kit an essential tool for studying collagen-related disorders and developing potential therapies.
Whether you are investigating musculoskeletal disorders, skin conditions, or other health issues in canine patients, the Canine Collagen Type I Alpha 2 ELISA Kit provides accurate and valuable data to support your research efforts. Trust in this kit to deliver reliable results and advance your understanding of collagen biology in dogs.
Product Name:
Canine Collagen Type I Alpha 2 (COL1a2) ELISA Kit
Product Code:
CNDL00021
Size:
96 Assays
Target:
COL1a2
Synonyms:
COL1-A2, OI4, Collagen Alpha-2(I)chain, osteogenesis imperfecta type IV, Collagen Of Skin,Tendon And bone,Alpha-2 Chain
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
