The CAMP (Cathelicidin Antimicrobial Peptide) ELISA Kit is a valuable tool for researchers looking to accurately measure levels of CAMP in various biological samples. This kit offers exceptional sensitivity and specificity, allowing for precise and reliable results for a variety of applications.CAMP is an important component of the innate immune system, playing a key role in defense against microbial pathogens. Its levels have been linked to various inflammatory and infectious diseases, making it a valuable biomarker for studying immune responses and developing new therapeutic strategies.
With easy-to-follow instructions and quick assay times, the CAMP ELISA Kit from Assay Genie is an indispensable resource for researchers working in immunology, infectious diseases, and antimicrobial research. Order yours today and enhance your research efforts with accurate measurements of CAMP levels.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with cAMP. During the reaction, cAMP in the sample or standard competes with a fixed amount of cAMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to cAMP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of cAMP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
85-100
86-98
Average (%)
94
92
92
1:4
Range (%)
91-103
87-101
101-114
Average (%)
97
92
107
1:8
Range (%)
84-98
90-102
99-116
Average (%)
91
97
107
1:16
Range (%)
86-100
90-102
99-114
Average (%)
92
97
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
95
EDTA plasma (n=5)
86-100
92
Cell culture media (n=5)
87-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.4
14.1
37.4
5.9
15.0
34.9
Standard deviation
0.3
0.7
2.0
0.4
0.9
1.8
C V (%)
5.56
4.96
5.35
6.78
6.0
5.16
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of cAMP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes cAMP in samples. No significant cross-reactivity or interference between cAMP and analogues was observed.
