Bovine Insulin Like Growth Factor Binding Protein 5 (IGFBP5) ELISA Kit
The Bovine Insulin-like Growth Factor Binding Protein 5 (IGFBP5) ELISA Kit is a powerful tool for researchers looking to accurately measure IGFBP5 levels in bovine serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it ideal for a variety of research applications.IGFBP5 is a key protein involved in regulating the biological activity of insulin-like growth factors, playing a crucial role in cell growth and differentiation.
Dysregulation of IGFBP5 has been linked to various diseases, including cancer and metabolic disorders, making it a valuable biomarker for studying these conditions and developing potential therapies.Overall, the Bovine IGFBP5 ELISA Kit offers researchers a precise and efficient way to analyze IGFBP5 levels, providing valuable insights into cellular processes and disease mechanisms in bovine species.
Product Name:
Bovine Insulin Like Growth Factor Binding Protein 5 (IGFBP5) ELISA Kit
Product Code:
BODL00084
Size:
96 Assays
Target:
IGFBP5
Synonyms:
IBP5
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
