Bovine Heat Shock 70kDa Protein 1B (HSPA1B) ELISA Kit
The Bovine Heat Shock 70kDa Protein 1B (HSPA1B) ELISA Kit offered by Assay Genie is a powerful tool for detecting and quantifying levels of HSPA1B in bovine serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring accurate and reliable results for researchers in a variety of fields.HSPA1B is an essential protein involved in the cellular stress response, playing a critical role in protecting cells from damage and promoting cell survival during times of stress.
Dysregulation of HSPA1B has been implicated in a variety of diseases, making it a valuable biomarker for studying conditions such as cancer, neurodegenerative disorders, and cardiovascular diseases.With the Assay Genie Bovine Heat Shock 70kDa Protein 1B (HSPA1B) ELISA Kit, researchers can confidently measure HSPA1B levels in bovine samples, advancing their understanding of cellular stress responses and potentially identifying new therapeutic targets.
Product Name:
Bovine Heat Shock 70kDa Protein 1B (HSPA1B) ELISA Kit
Product Code:
BODL00072
Size:
96 Assays
Target:
HSPA1B
Synonyms:
HSP70-2, HSP-A1B, Heat Shock Protein 72
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
