Bovine Cluster Of Differentiation 40 Ligand (CD40L) ELISA Kit

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SKU:
BODL00030
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P51749
Sensitivity:
0.18ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
CD154,TRAP,HIGM1,IGM,IMD3,TBAM,T-BAM,TNFSF5,Gp39,TNF Superfamily Member 5,Hyper-IgM Syndrome,TNF-Related Activation Protein,T-Cell B-Cell Activating Molecule
Reactivity:
Bovine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
Frequently bought together:

Description

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Bovine Cluster Of Differentiation 40 Ligand (CD40L) ELISA Kit

The Bovine Cluster of Differentiation 40 Ligand (CD40L) ELISA Kit is a reliable and accurate tool for detecting CD40L levels in bovine serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.CD40L is a key molecule in the immune system, playing a crucial role in mediating immune responses and inflammation. Dysregulation of CD40L is associated with various immune disorders and inflammatory conditions, making it a valuable biomarker for studying these diseases and developing targeted therapies.

Overall, the Bovine CD40L ELISA Kit provides researchers with a powerful tool for investigating the role of CD40L in immune responses and inflammatory diseases in bovine models, ultimately leading to a better understanding of these conditions and potential therapeutic interventions.

Product Name:Bovine Cluster Of Differentiation 40 Ligand (CD40L) ELISA Kit
Product Code:BODL00030
Size:96 Assays
Target:CD40L
Synonyms:CD154, TRAP, HIGM1, IGM, IMD3, TBAM, T-BAM, TNFSF5, Gp39, TNF Superfamily Member 5, Hyper-IgM Syndrome, TNF-Related Activation Protein, T-Cell B-Cell Activating Molecule
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)91-9894
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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