Anti-BIN2 항체의 (PACO56736)

Antibody Name:	BIN2 Antibody (PACO56736)
Antibody SKU:	PACO56736
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:20-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Bridging integrator 2 protein (290-496AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Immunofluorescence staining of HepG2 cells with PACO56736 at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	IHC image of PACO56736 diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
	IHC image of PACO56736 diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:	Promotes cell motility and migration, probably via its interaction with the cell membrane and with podosome proteins that mediate interaction with the cytoskeleton. Modulates membrane curvature and mediates membrane tubulation. Plays a role in podosome formation. Inhibits phagocytosis.
Synonyms:	Bridging integrator 2 (Breast cancer-associated protein 1), BIN2, BRAP1

UniProt Protein Function:	Promotes cell motility and migration, probably via its interaction with the cell membrane and with podosome proteins that mediate interaction with the cytoskeleton. Modulates membrane curvature and mediates membrane tubulation. Plays a role in podosome formation. Inhibits phagocytosis.
UniProt Protein Details:
NCBI Summary:
UniProt Code:	Q9UBW5
NCBI GenInfo Identifier:	143811367
NCBI Gene ID:	51411
NCBI Accession:	Q9UBW5.3
UniProt Secondary Accession:	Q9UBW5,Q86VV0, Q9NWK4, Q9UKN4
UniProt Related Accession:	Q9UBW5
Molecular Weight:	58,030 Da
NCBI Full Name:	Bridging integrator 2
NCBI Synonym Full Names:	bridging integrator 2
NCBI Official Symbol:	BIN2
NCBI Official Synonym Symbols:	BRAP-1
NCBI Protein Information:	bridging integrator 2
UniProt Protein Name:	Bridging integrator 2
UniProt Synonym Protein Names:	Breast cancer-associated protein 1
Protein Family:	Bridging integrator
UniProt Gene Name:	BIN2
UniProt Entry Name:

Antibodies

Secondary Antibody