Beta-Glucosidase Activity Assay Kit (BA0018)
- SKU:
- BA0018
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Sample Type:
- Biological
- Research Area:
- Clinical Chemistry
img:cited-250-x-100-px-125-x-75-px-.png
Frequently bought together:
Description
beta-Glucosidase Activity Assay Kit - Information
Assay Genie's beta-Glucosidase Assay Kit is designed to measure beta-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-beta-D-glucopyranoside that is hydrolyzed specifically by beta-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.Applications
For quantitative determination of beta-glucosidase activity and evaluation of drug effects on its metabolism.beta-Glucosidase Activity Assay Kit - Key Features
- High sensitivity and wide linear range. Use 20 sample. The detection limit is 2 U/L, linear up to 250 U/L.
- Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of beta-glucosidase activity within 20 minutes.
- Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.
beta-Glucosidase Activity Assay Kit - Data Sheet | |
| Kit Includes | Assay Buffer: 24 mL (pH 7.0) b-NPG Substrate: 1 mL Calibrator: 10 mL (equivalent to 250 U/L) |
| Kit Requires | Pipeting devices and accessories (e.g. multi-channel pipettor). Clear bottom 96-well plates and plate reader. |
| Method of Detection | OD405nm |
| Detection Limit | 2 U/L |
| Samples | Biological |
| Species | All |
| Protocol Length | 20 min |
| Size | 100 tests |
| Storage | Store all components at -20°C. |
| Shelf Life | 6 months |
More Details
beta-GLUCOSIDASE is a glucosidase enzyme which acts upon beta1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). beta-Glucosidases are required by organisms (some fungi, bacteria, termites) for consumption of cellulose. Lysozyme is also a beta-glucosidase and is present in tears to prevent bacterial infection of the eye. In humans, lower activity of a beta-glucosidase isoform (lysosomal gluco-cerebrosidase) has been related to Gaucher's disease and Parkinson's disease. Simple, direct and automation-ready procedures for measuring beta-glucosidase activity are becoming popular in Research and Drug Discovery.Citations
| Gao et al. | α-bisabolol exerts anti-inflammatory action and ameliorates collagen-induced arthritis in rats | Indian Journal of Animal Research 2022 | View Citation |
Related Products
img:cited-250-x-100-px-125-x-75-px-.png
Beta-N-Acetylglucosaminidase Activity Assay Kit (BA0053)
Estrogen Receptor-beta Transcription Factor Activity Assay
Human GBA3 / Cytosolic beta-glucosidase ELISA Kit
