The BB (Bilirubin) ELISA Kit is specifically designed for the precise measurement of bilirubin levels in human serum and plasma samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for researchers and healthcare professionals.Bilirubin is a key biomarker for liver health and function, with elevated levels indicating potential liver disease or dysfunction. Monitoring bilirubin levels is essential for diagnosing and managing liver conditions, as well as assessing the effectiveness of treatment strategies.
Whether conducting basic research studies or clinical investigations, the BB (Bilirubin) ELISA Kit provides a reliable and convenient solution for analyzing bilirubin levels in a variety of biological samples. Trust in this kit for robust and consistent results in your bilirubin studies.
Product Name:
Bb (Bilirubin) ELISA Kit (UNES00049)
SKU:
UNES00049
Target:
Bb (Bilirubin) (UNES00049)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.19 μg/mL
Detection range:
0.31-20 μg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bb. During the reaction, Bb in the sample or standard competes with a fixed amount of Bb on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Bb. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Bb in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
96-112
94-108
Average (%)
93
102
102
1:4
Range (%)
99-112
91-103
92-104
Average (%)
106
96
97
1:8
Range (%)
100-117
91-105
88-101
Average (%)
107
96
94
1:16
Range (%)
95-109
91-107
84-100
Average (%)
103
98
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-103
94
EDTA plasma (n=5)
88-102
94
Cell culture media (n=5)
90-107
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.9
11.5
45.4
5.1
10.6
48.2
Standard deviation
0.3
0.7
2.1
0.3
0.4
2.5
C V (%)
6.12
6.09
4.63
5.88
3.77
5.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Bb concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Bb in samples. No significant cross-reactivity or interference between Bb and analogues was observed.
