ATF1 (Phospho-Ser63) Colorimetric Cell-Based ELISA Kit

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SKU:
CBCAB01264
Product Type:
ELISA Kit
ELISA Type:
Cell Based Phospho Specific
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human
Mouse
Detection Method:
Colorimetric
Frequently bought together:

Description

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ATF1 (Phospho-Ser63)Colorimetric Cell-Based ELISA Kit

The ATF1 Phospho-Ser63 Colorimetric Cell-Based ELISA Kit is a powerful tool for the accurate detection of ATF1 phosphorylation at serine 63 in cell samples. This kit offers high sensitivity and specificity, providing researchers with reliable and reproducible results for a variety of research applications.ATF1 is a key transcription factor that plays a critical role in regulating gene expression in response to various stimuli, including stress and growth factors. Phosphorylation of ATF1 at serine 63 is known to enhance its transcriptional activity, making it an important marker for studying signaling pathways and cellular responses.

Whether studying signal transduction pathways, investigating cellular stress responses, or exploring the role of ATF1 in disease development, the ATF1 Phospho-Ser63 Colorimetric Cell-Based ELISA Kit is an essential tool for researchers looking to accurately measure ATF1 phosphorylation levels in cell samples.

Product Name:ATF1 (Phospho-Ser63) Colorimetric Cell-Based ELISA
Product Code:CBCAB01264
ELISA Type:Cell-Based
Target:ATF1 (Phospho-Ser63)
Reactivity:Human, Mouse
Dynamic Range:> 5000 Cells
Detection Method:Colorimetric 450 nm
Format:2 x 96-Well Microplates

The ATF1 (Phospho-Ser63) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect ATF1 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated ATF1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ATF1 phosphorylation.

Qualitative determination of ATF1 (Phospho-Ser63) concentration is achieved by an indirect ELISA format. In essence, ATF1 (Phospho-Ser63) is captured by ATF1 (Phospho-Ser63)-specific primary (1ø) antibodies while the HRP-conjugated secondary (2ø) antibodies bind the Fc region of the 1ø antibody. Through this binding, the HRP enzyme conjugated to the 2ø antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values.
2. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Database Information:Gene ID: 466, UniProt ID: P18846, OMIM: 123803/612160, Unigene: Hs.648565
Gene Symbol:ATF1
Sub Type:Phospho
UniProt Protein Function:ATF-1: a transcription factor that is a member of the leucine zipper family. Forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. Binds the Tax-responsive element (TRE) of HTLV-I. Activated downstream of IL-1. c-Src and TRAF6 are mediators of IL-1-induced AP-1 activation. Plays an important role in the trans-activation of the MHC class II trans-activator (CIITA) promoter III in B cells.
UniProt Protein Details:

Protein type:Transcription factor

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; nucleus

Molecular Function:protein binding; protein complex binding; protein heterodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; transcription factor activity

Biological Process: cellular protein complex assembly; innate immune response; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of DNA replication; positive regulation of transcription from RNA polymerase II promoter; response to cobalt ion; response to organic cyclic substance; stress-activated MAPK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; transcription from RNA polymerase II promoter

NCBI Summary:This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6. [provided by RefSeq, Aug 2010]
UniProt Code:P18846
NCBI GenInfo Identifier:1168542
NCBI Gene ID:466
NCBI Accession:P18846.2
UniProt Secondary Accession:P18846,P25168, Q9H4A8, B4DRF9,
UniProt Related Accession:P18846,AAB25878
Molecular Weight:15,295 Da
NCBI Full Name:Cyclic AMP-dependent transcription factor ATF-1
NCBI Synonym Full Names:activating transcription factor 1
NCBI Official Symbol:ATF1  
NCBI Official Synonym Symbols:TREB36; EWS-ATF1; FUS/ATF-1  
NCBI Protein Information:cyclic AMP-dependent transcription factor ATF-1
UniProt Protein Name:Cyclic AMP-dependent transcription factor ATF-1
UniProt Synonym Protein Names:Activating transcription factor 1; Protein TREB36
Protein Family:Alcohol O-acetyltransferase
UniProt Gene Name:ATF1  
UniProt Entry Name:ATF1_HUMAN
ComponentQuantity
96-Well Cell Culture Clear-Bottom Microplate2 plates
10X TBS24 mL
Quenching Buffer24 mL
Blocking Buffer50 mL
15X Wash Buffer50 mL
Primary Antibody Diluent12 mL
100x Anti-Phospho Target Antibody 60 µL
100x Anti-Target Antibody60 µL
Anti-GAPDH Antibody60 µL
HRP-Conjugated Anti-Rabbit IgG Antibody12 mL
HRP-Conjugated Anti-Mouse IgG Antibody12 mL
SDS Solution12 mL
Stop Solution24 mL
Ready-to-Use Substrate12 mL
Crystal Violet Solution12 mL
Adhesive Plate Seals2 seals

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)
  • Deionized or sterile water

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1.Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37 °C prior to adding cells.
2.Incubate the cells for overnight at 37 °C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice.
5.Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4 °C for a week.
7.Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time.
9.Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
11.Add 50 µL of 1x primary antibodies Anti-ATF1 (Phospho-Ser63) Antibody, Anti-ATF1 Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4 °C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
13.Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature.
14.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
15.Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark.
16.Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader.

(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)

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