Aspartate Transaminase (AST) Activity Assay Kit (BA0079)

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SKU:
BA0079
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma
Research Area:
Enzyme Activity
Clinical Chemistry
Frequently bought together:

Description

ELISA Kit Technical ManualMSDS

Aspartate Transaminase Activity Assay Kit - Information

Assay Genie's Aspartate Transaminase (AST), also known as serum glutamic oxaloacetic transaminase (GOT) or aspartate aminotransferase (ASAT/AAT), facilitates the conversion of aspartate and a-ketoglutarate to oxaloacetate and glutamate. There are two isoenzymes in humans: GOT1 is a cytosolic isoenzyme derived from red blood cells and heart; GOT2 is the mitochondrial isoenzyme found mainly in the liver. AST is elevated in liver and muscle diseases. It is part of diagnostic tests for liver function, myocardial infarction, acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease and trauma. Simple, direct and automation-ready procedures for measuring AST activity find wide applications in research and drug discovery.AST activity assay is based on the quantification of oxaloacetate produced by AST. In this assay, oxaloacetate and NADH are converted to malate and NAD by the enzyme malate dehydrogenase. The decrease in NADH absorbance at 340 nm is proportionate to AST activity.

Applications

For quantitative determination of aspartate transaminase (AST) activity and evaluation of drug effects on AST activity.

Aspartate Transaminase Activity Assay Kit - Key Features

  • Sensitive. Linear detection range: 2-100 U/L.
  • Simple and convenient. This simple, convenient assay can be carried out in a microplate or a cuvette and takes only 10 min

Aspartate Transaminase Activity Assay Kit -Data Sheet

Kit IncludesDeveloper: 10 mL AST Enzyme: 240 mL Dye Reagent: 120 mL Cosubstrate: 600 mL ODC Enzyme: 120 mL Aspartate Standard: 400 mL
Kit RequiresPipetting devices, clear or black flat-bottom 96-well plates, plate reader or centrifuge tubes.
Method of DetectionOD340nm
Detection Limit2 U/L
SamplesSerum, plasma etc
SpeciesAll
Protocol Length10 min
Size100 tests
StorageStore all kit components at -20 °C
Shelf Life6 months

More Details

Aspartate Transaminase (AST), also known as serum glutamic oxaloacetic transaminase (GOT) or aspartate aminotransferase (ASAT/AAT), facilitates the conversion of aspartate and a-ketoglutarate to oxaloacetate and glutamate. There are two isoenzymes in humans: GOT1 is a cytosolic isoenzyme derived from red blood cells and heart; GOT2 is the mitochondrial isoenzyme found mainly in the liver. AST is elevated in liver and muscle diseases. It is part of diagnostic tests for liver function, myocardial infarction, acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease and trauma. Simple, direct and automation-ready procedures for measuring AST activity find wide applications in research and drug discovery.

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