Aspartate Assay Kit (BA0078)
- SKU:
- BA0078
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Sample Type:
- Plasma, Serum, Tissue, Culture Media
- Research Area:
- Urea
- Amino Acid and Protein
- Clinical Chemistry
Frequently bought together:
Description
Aspartate Assay Kit - Information
Assay Genie's Aspartate Assay Kit provides a simple, direct and automation-ready procedure for measuring aspartate concentration. Aspartate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity of the oxidized dye at 570 nm or fluorescence intensity at Ex/Em = 530/585 nm is directly proportional to the aspartate concentration in the sample.Applications
For quantitative determination of aspartate and evaluation of drug effects on aspartate metabolism.Aspartate Assay Kit - Key Features
- Sensitive and accurate. Linear detection range: 2 to 400 aspartate for colorimetric assays and 1 to 50 for fluorimetric assays.
Aspartate Assay Kit - Data Sheet | |
Kit Includes | Developer: 10 mL AST Enzyme: 240 mL Dye Reagent: 120 mL Cosubstrate: 600 mL ODC Enzyme: 120 mL Aspartate Standard: 400 mL |
Kit Requires | Pipetting devices, clear or black flat-bottom 96-well plates, plate reader or centrifuge tubes. |
Method of Detection | OD570nm, or FL530/585nm |
Detection Limit | OD, FL: 2, 1 |
Samples | Plasma, serum, tissue, culture media etc |
Species | All |
Protocol Length | 60 min |
Size | 100 tests |
Storage | Store all kit components at -20 °C. |
Shelf Life | 6 months |