Anti-ZWINT 항체 (CAB6328)

Antibody Name:	Anti-ZWINT Antibody
Antibody SKU:	CAB6328
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-277 of human ZWINT (NP_008988.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, SW620, HepG2, Jurkat, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-277 of human ZWINT (NP_008988.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEAA ETEA EAAA LEVL AEVA GILE PVGL QEEA ELPA KILV EFVV DSQK KDKL LCSQ LQVA DFLQ NILA QEDT AKGL DPLA SEDT SRQK AIAA KEQW KELK ATYR EHVE AIKI GLTK ALTQ MEEA QRKR TQLR EAFE QLQA KKQM AMEK RRAV QNQW QLQQ EKHL QHLA EVSA EVRE RKTG TQQE LDRV FQKL GNLK QQAE QERD KLQR YQTF LQLL YTLQ GKLL FPEA EAEA ENLP DDKP QQPT RPQE QSTG DTMG RDPG VSFK AVGL QPAG DVNL P
Gene ID:	11130
Uniprot:	O95229
Cellular Location:	Chromosome, Nucleus, centromere, kinetochore
Calculated MW:	25kDa/31kDa
Observed MW:	39kDa

Synonyms:

ZWINT, HZwint-1, KNTC2AP, SIP30, ZWINT1

Background:

This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	ZWINT: Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.
UniProt Protein Details:	Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 10q21-q22 Cellular Component: cytoplasm; cytosol; kinetochore Molecular Function:protein binding; protein N-terminus binding Biological Process: establishment of cellular localization; mitotic cell cycle checkpoint; mitotic sister chromatid segregation; sister chromatid cohesion
NCBI Summary:	This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	O95229
NCBI GenInfo Identifier:	117949386
NCBI Gene ID:	11130
NCBI Accession:	O95229.2
UniProt Secondary Accession:	O95229,Q0D2I3, Q9BWD0, A6NNV6,
UniProt Related Accession:	O95229
Molecular Weight:	25,544 Da
NCBI Full Name:	ZW10 interactor
NCBI Synonym Full Names:	ZW10 interacting kinetochore protein
NCBI Official Symbol:	ZWINT
NCBI Official Synonym Symbols:	SIP30; ZWINT1; KNTC2AP; HZwint-1
NCBI Protein Information:	ZW10 interactor
UniProt Protein Name:	ZW10 interactor
UniProt Synonym Protein Names:	ZW10-interacting protein 1; Zwint-1
Protein Family:	ZW10 interactor
UniProt Gene Name:	ZWINT
UniProt Entry Name:	ZWINT_HUMAN

	Western blot analysis of extracts of various cell lines, using ZWINT antibody (CAB6328) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of C6 cells using ZWINT Polyclonal Antibody (CAB6328) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using ZWINT Polyclonal Antibody (CAB6328) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using ZWINT Polyclonal Antibody (CAB6328) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)