Anti-ZNRF1 Antibody (CAB15918)
- SKU:
- CAB15918
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| Antibody Name: | Anti-ZNRF1 Antibody |
| Antibody SKU: | CAB15918 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human ZNRF1 (NP_115644.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, Jurkat, Mouse thymus, Mouse lung, Rat thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human ZNRF1 (NP_115644.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGGK QSTA ARSR GPFP GVST DDSA VPPP GGAP HFGH YRTG GGAM GLRS RSVS SVAG MGMD PSTA GGVP FGLY TPAS RGTG DSER APGG GGSA SDST YAHG NGYQ ETGG GHHR DGML YLGS RASL ADAL PLHI APRW FSSH SGFK CPIC SKSV ASDE MEMH FIMC LSKP RL |
| Gene ID: | 84937 |
| Uniprot: | Q8ND25 |
| Cellular Location: | Cytoplasmic vesicle, Endosome, Lysosome, Membrane, Peripheral membrane protein, secretory vesicle, synaptic vesicle membrane |
| Calculated MW: | 23kDa/29kDa |
| Observed MW: | 24kDa |
| Synonyms: | ZNRF1, NIN283 |
| Background: | This gene encodes an E3 ubiquitin-protein ligase that plays a role in neural-cell differentiation. Overexpression of this gene causes neurite-like elongation. The encoded protein contains both a zinc finger and a RING finger motif and is localized in the endosome/lysosome compartment, indicating that it may be involved in ubiquitin-mediated protein modification, and in synaptic vessicle membranes in neurons. |
| UniProt Protein Function: | ZNRF1: E3 ubiquitin-protein ligase that mediates the ubiquitination of AKT1 and GLUL, thereby playing a role in neuron cells differentiation. Plays a role in the establishment and maintenance of neuronal transmission and plasticity. Regulates Schwann cells differentiation by mediating ubiquitination of GLUL. Promotes neurodegeneration by mediating 'Lys-48'-linked polyubiquitination and subsequent degradation of AKT1 in axons: degradation of AKT1 prevents AKT1-mediated phosphorylation of GSK3B, leading to GSK3B activation and phosphorylation of DPYSL2/CRMP2 followed by destabilization of microtubule assembly in axons (Probable). 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ligase; EC 6.3.2.19; Ubiquitin ligase; EC 6.3.2.-; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 16q23.1 Cellular Component: cytosol; endosome; membrane Molecular Function:protein binding; ubiquitin-protein ligase activity Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination |
| NCBI Summary: | This gene encodes an E3 ubiquitin-protein ligase that plays a role in neural-cell differentiation. Overexpression of this gene causes neurite-like elongation. The encoded protein contains both a zinc finger and a RING finger motif and is localized in the endosome/lysosome compartment, indicating that it may be involved in ubiquitin-mediated protein modification, and in synaptic vessicle membranes in neurons. [provided by RefSeq, Feb 2012] |
| UniProt Code: | Q8ND25 |
| NCBI GenInfo Identifier: | 126253846 |
| NCBI Gene ID: | 84937 |
| NCBI Accession: | Q8ND25.2 |
| UniProt Secondary Accession: | Q8ND25,Q9H083, D3DUJ9, |
| UniProt Related Accession: | Q8ND25 |
| Molecular Weight: | 29,777 Da |
| NCBI Full Name: | E3 ubiquitin-protein ligase ZNRF1 |
| NCBI Synonym Full Names: | zinc and ring finger 1, E3 ubiquitin protein ligase |
| NCBI Official Symbol: | ZNRF1 |
| NCBI Official Synonym Symbols: | NIN283 |
| NCBI Protein Information: | E3 ubiquitin-protein ligase ZNRF1 |
| UniProt Protein Name: | E3 ubiquitin-protein ligase ZNRF1 |
| UniProt Synonym Protein Names: | Nerve injury-induced gene 283 protein; Zinc/RING finger protein 1 |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | ZNRF1 |
| UniProt Entry Name: | ZNRF1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ZNRF1 antibody (CAB15918) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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