Anti-ZNF189 Antibody (CAB16446)

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SKU:
CAB16446
Product type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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Antibody Name:Anti-ZNF189 Antibody
Antibody SKU:CAB16446
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human ZNF189 (NP_001265160.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human ZNF189 (NP_001265160.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MASP SPPP ESKE EWDY LDPA QRSL YKDV MMEN YGNL VSLD VLNR DKDE EPTV KQEI EEIE EEVE PQGV IVTR IKSE IDQD PMGR ETFE LVGR LDKQ RGIF LWEI PRES LTQE QRMF RENT NIIR KRPN SEEK CHKC EECG KGFV RKAH FI
Gene ID:7743
Uniprot:O75820
Cellular Location:Nucleus
Calculated MW:62kDa/68kDa/71kDa/72kDa
Observed MW:73kDa
Synonyms:ZNF189
Background:Kruppel-like zinc finger proteins such as ZNF189 contain a conserved stretch of 7 amino acids that connects a variable number of DNA-binding zinc finger repeats of the cys(2)his(2) (C2H2) type. Approximately 30% of human Kruppel-like zinc finger proteins contain an N-terminal Kruppel-associated box (KRAB) domain. The KRAB domain consists of approximately 75 amino acids that may be subdivided into an A box, which is present in every KRAB domain and is essential for transcriptional repression, and a B box, which is not always present.
NCBI Summary:Kruppel-like zinc finger proteins such as ZNF189 contain a conserved stretch of 7 amino acids that connects a variable number of DNA-binding zinc finger repeats of the cys(2)his(2) (C2H2) type (summarized by Odeberg et al., 1998 [PubMed 9653648]). Approximately 30% of human Kruppel-like zinc finger proteins contain an N-terminal Kruppel-associated box (KRAB) domain. The KRAB domain consists of approximately 75 amino acids that may be subdivided into an A box, which is present in every KRAB domain and is essential for transcriptional repression, and a B box, which is not always present.[supplied by OMIM, May 2010]
UniProt Code:O75820
NCBI GenInfo Identifier:37693525
NCBI Gene ID:7743
NCBI Accession:NP_003443
UniProt Related Accession:O75820
Molecular Weight:73kDa
NCBI Full Name:zinc finger protein 189 isoform 1
NCBI Synonym Full Names:zinc finger protein 189
NCBI Official Symbol:ZNF189  
NCBI Protein Information:zinc finger protein 189
UniProt Protein Name:Zinc finger protein 189
Protein Family:Zinc finger protein
UniProt Gene Name:ZNF189  
UniProt Entry Name:ZN189_HUMAN
Anti-ZNF189 Antibody (CAB16446)Western blot analysis of extracts of Jurkat cells, using ZNF189 antibody (CAB16446) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

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