Anti-ZEB1 Antibody (CAB5600)
- SKU:
- CAB5600
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-ZEB1 Antibody |
| Antibody SKU: | CAB5600 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ZEB1 (NP_001167567.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SKOV3, HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ZEB1 (NP_001167567.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MADG PRCK RRKQ ANPR RNNV TNYN TVVE TNSD SDDE DKLH IVEE ESVT DAAD CEGV PEDD LPTD QTVL PGRS SERE GNAK NCWE DDTG KEGQ EILG PEAQ ADEA GCTV KDDE CESD AENE QNHD PNVE EFLQ QQDT AVIF PEAP EEDQ RQGT PEAS GHDE NGTP DAFS QLLT CPYC DRGY KRFT SLKE HIKY RHEK NEDN FSCS LCSY TFAY RTQL ERHM TSHK SGRD QRHV TQSG CNRK FKCT ECGK AFKY KHHL KEHL |
| Gene ID: | 6935 |
| Uniprot: | P37275 |
| Cellular Location: | Nucleus |
| Calculated MW: | 116kDa/122kDa/124kDa |
| Observed MW: | 124kDa |
| Synonyms: | ZEB1, AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP, ZFHX1A |
| Background: | This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described. |
| UniProt Protein Function: | TCF8: Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. Interacts (via N-terminus) with SMARCA4/BRG1. Colocalizes with SMARCA4/BRG1 in E-cadherin- negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas. Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family. |
| UniProt Protein Details: | Protein type:C2H2-type zinc finger protein; DNA-binding; Transcription, coactivator/corepressor; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 10p11.2 Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus Molecular Function:protein binding; zinc ion binding; double-stranded DNA binding; transcription coactivator activity; chromatin binding; transcription factor binding; transcription factor activity; transcription corepressor activity Biological Process: response to nutrient levels; transcription, DNA-dependent; regulation of T cell differentiation in the thymus; semicircular canal morphogenesis; negative regulation of epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; pattern specification process; embryonic skeletal morphogenesis; regulation of transcription from RNA polymerase II promoter; regulation of mesenchymal cell proliferation; cell proliferation; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; cartilage development; regulation of smooth muscle cell differentiation; forebrain development; positive regulation of transcription from RNA polymerase II promoter; immune response; response to activity; embryonic camera-type eye morphogenesis; negative regulation of transcription, DNA-dependent; positive regulation of neuron differentiation Disease: Corneal Dystrophy, Posterior Polymorphous, 3; Corneal Dystrophy, Fuchs Endothelial, 6 |
| NCBI Summary: | This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010] |
| UniProt Code: | P37275 |
| NCBI GenInfo Identifier: | 6166575 |
| NCBI Gene ID: | 6935 |
| NCBI Accession: | P37275.2 |
| UniProt Secondary Accession: | P37275,Q12924, Q13800, Q2KJ05, Q5T968, Q5VZ84, Q8NB68 B4DJV0, B4DUW9, E9PCM7, F5H4I8, |
| UniProt Related Accession: | P37275 |
| Molecular Weight: | 122,084 Da |
| NCBI Full Name: | Zinc finger E-box-binding homeobox 1 |
| NCBI Synonym Full Names: | zinc finger E-box binding homeobox 1 |
| NCBI Official Symbol: | ZEB1 |
| NCBI Official Synonym Symbols: | BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1 |
| NCBI Protein Information: | zinc finger E-box-binding homeobox 1; negative regulator of IL2; delta-crystallin enhancer binding factor 1; posterior polymorphous corneal dystrophy 3; zinc finger homeodomain enhancer-binding protein; transcription factor 8 (represses interleukin 2 expression) |
| UniProt Protein Name: | Zinc finger E-box-binding homeobox 1 |
| UniProt Synonym Protein Names: | NIL-2-A zinc finger protein; Negative regulator of IL2; Transcription factor 8; TCF-8 |
| Protein Family: | Zinc finger E-box-binding homeobox |
| UniProt Gene Name: | ZEB1 |
| UniProt Entry Name: | ZEB1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ZEB1 antibody (CAB5600) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human mammary cancer using ZEB1 antibody (CAB5600) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat testis using ZEB1 antibody (CAB5600) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using ZEB1 antibody (CAB5600) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using ZEB1 antibody (CAB5600). Blue: DAPI for nuclear staining. |
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