Anti-YWHAQ Antibody (CAB2563)
- SKU:
- CAB2563
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-YWHAQ Antibody |
| Antibody SKU: | CAB2563 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human YWHAQ (NP_006817.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SW480, BT-474, HeLa, Jurkat, Mouse liver, Mouse kidney, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human YWHAQ (NP_006817.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEKT ELIQ KAKL AEQA ERYD DMAT CMKA VTEQ GAEL SNEE RNLL SVAY KNVV GGRR SAWR VISS IEQK TDTS DKKL QLIK DYRE KVES ELRS ICTT VLEL LDKY LIAN ATNP ESKV FYLK MKGD YFRY LAEV ACGD DRKQ TIDN SQGA YQEA FDIS KKEM QPTH PIRL GLAL NFSV FYYE ILNN PELA CTLA KTAF DEAI AELD TLNE DSYK DSTL IMQL LRDN LTLW TSDS AGEE CDAA EGAE N |
| Gene ID: | 10971 |
| Uniprot: | P27348 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 27kDa |
| Observed MW: | 32kDa |
| Synonyms: | YWHAQ, 14-3-3, 1C5, HS1 |
| Background: | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. |
| UniProt Protein Function: | 14-3-3 theta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Associates with c-BCR and BCR-Abl. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 2p25.1 Cellular Component: cytoplasm; cytoplasmic vesicle membrane; cytosol; focal adhesion; membrane; mitochondrion; protein complex Molecular Function:protein binding; protein C-terminus binding; protein domain specific binding; protein N-terminus binding Biological Process: apoptosis; gene expression; negative regulation of transcription, DNA-dependent; programmed cell death; small GTPase mediated signal transduction; substantia nigra development; transcription initiation from RNA polymerase II promoter |
| NCBI Summary: | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P27348 |
| NCBI GenInfo Identifier: | 112690 |
| NCBI Gene ID: | 10971 |
| NCBI Accession: | P27348.1 |
| UniProt Secondary Accession: | P27348,Q567U5, Q5TZU8, Q9UP48, D6W4Z5, |
| UniProt Related Accession: | P27348 |
| Molecular Weight: | |
| NCBI Full Name: | 14-3-3 protein theta |
| NCBI Synonym Full Names: | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta |
| NCBI Official Symbol: | YWHAQ |
| NCBI Official Synonym Symbols: | 1C5; HS1; 14-3-3 |
| NCBI Protein Information: | 14-3-3 protein theta |
| UniProt Protein Name: | 14-3-3 protein theta |
| UniProt Synonym Protein Names: | 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1 |
| Protein Family: | 14-3-3 protein |
| UniProt Gene Name: | YWHAQ |
| UniProt Entry Name: | 1433T_HUMAN |
 | Western blot analysis of extracts of various cell lines, using YWHAQ antibody (CAB2563) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of HeLa cells using YWHAQ antibody (CAB2563). Blue: DAPI for nuclear staining. |